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2.3.11 FUTURE PROSPECTS ON RESEARCH OF HAAs IN HUMAN HEALTH RISK The analysis of HAA biomarkers in humans remains a challenging analytical task, because HAAs are present at the ppb levels in the diet. However, as the sensitivity of MS instrumentation continues to improve, the establishment of LC/MS-based methods to measure biomarkers such as urinary HAA metabolites, and HAA protein and HAA DNA adducts in human populations will become more facile. Future studies will require MS analysis of biomarkers of multiple HAAs, since the extent of exposure to various HAAs can vary in the diet. Through a combination of analyses for multiple urinary metabolites, HAA protein and HAA DNA adducts, and other biomarkers of longer-term exposures, such as HAA accumulation in hair (252, 253), it may be feasible to assess more reliably the exposure to HAAs and to determine the biologically effective dose of each HAA and the resultant potential genetic damage. With the identi cation of such biomarkers, the interactive effects of genetic polymorphisms of XMEs involved in HAA metabolism (activation and detoxication) will be able to be correlated with the levels of adduction products and cancer risk in human population studies. Such analyses should clarify the role of HAAs as a critical dietary factor in the initiation of colorectal and other common human cancers.

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1. Sugimura, T., Nagao, N., Kawachi, T., Honda, M., Yahagi, T., Seino, Y., Stao, S., Matsukura, N., Matsushima, T., Shirai, A., Sawamura, M., Matsumoto, H. (1977).

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Mutagen-carcinogens in food, with special reference to highly mutagenic pyrolytic products in broiled foods, in Origins of Human Cancer, Book C (eds HH Hiatt, JD Watson, JA Winstein), Cold Spring Harbour Laboratory, Cold Spring Harbour, New York, 1561 1577 Felton, JS, J gerstad, M, Knize, MG, Skog, K, Wakabayashi, K (2000) Contents in foods, beverages and tobacco, in Food Borne Carcinogens Heterocyclic Amines (eds M, Nagao, T, Sugimura), John Wiley & Sons, Ltd, Chichester, England, pp 31 71 Skog, KI, Johansson, MA, J gerstad, MI (1998) Carcinogenic heterocyclic amines in model systems and cooked foods: a review on formation, occurrence and intake Food Chem Toxicol, 36, 879 896 Sugimura, T, Wakabayashi, K, Nakagama, H, Nagao, M (2004) Heterocyclic amines: mutagens/carcinogens produced during cooking of meat and sh Cancer Sci.

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, 95, 290 299 Turesky, RJ, Goodenough, AK, Ni, W, McNaughton, L, LeMaster, DM, Holland, RD, Wu, RW, Felton, JS (2007) Identi cation of 2-Amino-1,7dimethylimidazo[4,5-g]quinoxaline: an abundant mutagenic heterocyclic aromatic amine formed in cooked beef Chem Res Toxicol, 20, 520 530 Manabe, S, Izumikawa, S, Asakuno, K, Wada, O, Kanai, Y (1991) Detection of carcinogenic amino-alpha-carbolines and amino-gamma-carbolines in dieselexhaust particles Environ Pollut, 70, 255 265 Manabe, S, Tohyama, K, Wada, O, Aramaki, T (1991) Detection of a carcinogen, 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine, in cigarette smoke condensate Carcinogenesis, 12, 1945 1947 National Toxicology Program (2005) Report on Carcinogenesis, 11th edn, US Department of Health and Human Services, Public Health Service, Research Triangle Park, NC Sugimura, T (1992) Multistep carcinogenesis: a 1992 perspective Science, 258, 603 607 Manabe, S, Wada, O.

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, Kanai, Y (1990) Simultaneous determination of aminoalpha-carbolines and amino-gamma-carbolines in cigarette smoke condensate by high-performance liquid chromatography J Chromatogr, 529, 125 133 Yoshida, D, Matsumoto, T (1980) Amino-alpha-carbolines as mutagenic agents in cigarette smoke condensate Cancer Lett, 10, 141 149 Hecht, SS (2003) Tobacco carcinogens, their biomarkers and tobacco-induced cancer Nat Rev Cancer, 3, 733 744 J gerstad, M, Skog, K, Grivas, S, Olsson, K (1991) Formation of heterocyclic amines using model systems Mutat Res, 259, 219 233 Murkovic, M (2004) Formation of heterocyclic aromatic amines in model systems J Chromatogr, B, 802, 3 10 Milic, BL, Djilas, SM, Candadanoic-Brunet, JM (1993) Synthesis of some heterocyclic aminoimidazoarenes Food Chem, 46, 273 276 Skog, K, J gerstad, M (1993).

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Incorporation of carbon atoms from glucose into the food mutagens MeIQx and 4,8-DiMeIQx using 14C-labelled glucose in a model system Carcinogenesis, 14, 2027 2031 Shioya, M, Wakabayashi, K, Sato, S, Nagao, M, Sugimura, T (1987) Formation of a mutagen, 2-amino-1-methyl-6-phenylimidazo[4,5-b]-pyridine (PhIP) in.

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cooked beef, by heating a mixture containing creatinine, phenylalanine and glucose Mutat Res, 191, 133 138 Pearson, AM, Chen, C, Gray, JI, Aust, SD (1992) Mechanism(s) involved in meat mutagen formation and inhibition Free Radic Biol Med, 13, 161 167 Kato, T, Harashima, T, Moriya, N, Kikugawa, K, Hiramoto, K (1996) Formation of the mutagenic/carcinogenic imidazoquinoxaline-type heterocyclic amines through the unstable free radical Maillard intermediates and its inhibition by phenolic antioxidants Carcinogenesis, 17, 2469 2476 Kikugawa, K, Kato, T, Hiramoto, K, Takada, C, Tanaka, M, Maeda, Y, Ishihara, T (1999) Participation of the pyrazine cation radical in the formation of mutagens in the reaction of glucose/glycine/creatinine Mutat Res, 444, 133 144 Kikugawa, K (1999) Involvement of free radicals in the formation of heterocyclic amines and prevention by antioxidants Cancer Lett.

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